Primers Available
Organism:Setaria digitata mitochondrion
Repeat:(T)10
Start:2081 End:2090
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CGTTTGATCTATTATTTGCGTGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCAAACAACGACCTTCAAACA | |||||||||||||
| Tm_F_1 | 60.705 | Size_F_1 | 23 | Tm_R_1 | 59.205 | Size_R_1 | 21 | PRODUCT Size | 271 bp | Primer Start | 1920 | Primer End | 2190 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCGTTTGATCTATTATTTGCGTG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCAAACAACGACCTTCAAACA | |||||||||||||
| Tm_F_2 | 59.186 | Size_F_2 | 23 | Tm_R_2 | 59.205 | Size_R_2 | 21 | PRODUCT Size | 272 bp | Primer Start | 1919 | Primer End | 2190 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTATTTGCGTGGTTATTTTAACG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGCAAACAAATCAAACAACG | |||||||||||||
| Tm_F_3 | 58.267 | Size_F_3 | 23 | Tm_R_3 | 59.062 | Size_R_3 | 20 | PRODUCT Size | 270 bp | Primer Start | 1931 | Primer End | 2200 |