Primers Available
Organism: Pisum sativum chloroplast
Repeat:(A)11tgattcttcttttcttcacctattatcgtttttcttagaacgaaacaatctcaataggattgtcggatttttct(A)10taccaatccgcataaactttgagtttagattcaattaatgaagtaacgtaactcta(T)16agaacaggagttctagttctagtaattgactttcttttttcata(T)10
Start:98282 End:98502
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCAAAATAAATAATCAACCACGC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTTTGTAAATTCGTCATCTTTTGTT | |||||||||||||
| Tm_F_1 | 58.141 | Size_F_1 | 23 | Tm_R_1 | 58.31 | Size_R_1 | 25 | PRODUCT Size | 279 bp | Primer Start | 98256 | Primer End | 98534 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCAAAATAAATAATCAACCACGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CTTTTGTAAATTCGTCATCTTTTG | |||||||||||||
| Tm_F_2 | 58.141 | Size_F_2 | 23 | Tm_R_2 | 57.209 | Size_R_2 | 24 | PRODUCT Size | 280 bp | Primer Start | 98256 | Primer End | 98535 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCAAAATAAATAATCAACCACGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CTTTTGTAAATTCGTCATCTTTTGTT | |||||||||||||
| Tm_F_3 | 58.141 | Size_F_3 | 23 | Tm_R_3 | 59.137 | Size_R_3 | 26 | PRODUCT Size | 280 bp | Primer Start | 98256 | Primer End | 98535 |