Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(TTTGT)3
Start:307816 End:307830
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACGAAACCTAGACGCAAGGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCCTTTTCCCCAGTTCAAAT | |||||||||||||
| Tm_F_1 | 59.875 | Size_F_1 | 20 | Tm_R_1 | 60.159 | Size_R_1 | 20 | PRODUCT Size | 223 bp | Primer Start | 307731 | Primer End | 307953 |
| FORWARD_PRIMER_2 | |||||||||||||
| ACGAAACCTAGACGCAAGGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCGCCTCATCAACAAACAAA | |||||||||||||
| Tm_F_2 | 59.875 | Size_F_2 | 20 | Tm_R_2 | 60.234 | Size_R_2 | 20 | PRODUCT Size | 196 bp | Primer Start | 307731 | Primer End | 307926 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACGAAACCTAGACGCAAGGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CGCCTCATCAACAAACAAAA | |||||||||||||
| Tm_F_3 | 59.875 | Size_F_3 | 20 | Tm_R_3 | 59.706 | Size_R_3 | 20 | PRODUCT Size | 195 bp | Primer Start | 307731 | Primer End | 307925 |