Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(TTTG)3agggtagactctctctcaagctcctactgattgaactccgtaaactcaatcacctatttcttcttgatcctttggc(TACT)3
Start:931624 End:931723
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CGTCAAGGTAGAGTCAAGAGGAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GAACCCCATTCATTTACCCA | |||||||||||||
| Tm_F_1 | 59.929 | Size_F_1 | 23 | Tm_R_1 | 59.481 | Size_R_1 | 20 | PRODUCT Size | 274 bp | Primer Start | 931550 | Primer End | 931823 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTCGTCAAGGTAGAGTCAAGAGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GAACCCCATTCATTTACCCA | |||||||||||||
| Tm_F_2 | 59.929 | Size_F_2 | 23 | Tm_R_2 | 59.481 | Size_R_2 | 20 | PRODUCT Size | 276 bp | Primer Start | 931548 | Primer End | 931823 |
| FORWARD_PRIMER_3 | |||||||||||||
| CGTCAAGGTAGAGTCAAGAGGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GAACCCCATTCATTTACCCA | |||||||||||||
| Tm_F_3 | 58.6 | Size_F_3 | 22 | Tm_R_3 | 59.481 | Size_R_3 | 20 | PRODUCT Size | 274 bp | Primer Start | 931550 | Primer End | 931823 |