Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(TTTC)5tcagtggtggtatacaatcggtttaggtactaaggggtatggatctttctttatacgggaggggagctcttctttttcta(TTCT)4
Start:297452 End:297567
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AAAGCTTTTACTCGAGGGGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ATTCGGCATTTTGAAACCAC | |||||||||||||
| Tm_F_1 | 59.72 | Size_F_1 | 20 | Tm_R_1 | 59.807 | Size_R_1 | 20 | PRODUCT Size | 245 bp | Primer Start | 297401 | Primer End | 297645 |
| FORWARD_PRIMER_2 | |||||||||||||
| AAAGCTTTTACTCGAGGGGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AGACGAGATTCGGCATTTTG | |||||||||||||
| Tm_F_2 | 59.72 | Size_F_2 | 20 | Tm_R_2 | 60.214 | Size_R_2 | 20 | PRODUCT Size | 252 bp | Primer Start | 297401 | Primer End | 297652 |
| FORWARD_PRIMER_3 | |||||||||||||
| AAAGCTTTTACTCGAGGGGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTTGAAACCACGAAAGGCTC | |||||||||||||
| Tm_F_3 | 59.72 | Size_F_3 | 20 | Tm_R_3 | 60.227 | Size_R_3 | 20 | PRODUCT Size | 236 bp | Primer Start | 297401 | Primer End | 297636 |