Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(TTTC)4
Start:944190 End:944205
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTTCTCTTGCCTGCTTGCTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTACCTCCGGAAATAAGGCA | |||||||||||||
| Tm_F_1 | 59.898 | Size_F_1 | 20 | Tm_R_1 | 59.542 | Size_R_1 | 20 | PRODUCT Size | 130 bp | Primer Start | 944099 | Primer End | 944228 |
| FORWARD_PRIMER_2 | |||||||||||||
| GAACCTCACTCCTGTCGGTC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTACCTCCGGAAATAAGGCA | |||||||||||||
| Tm_F_2 | 59.688 | Size_F_2 | 20 | Tm_R_2 | 59.542 | Size_R_2 | 20 | PRODUCT Size | 233 bp | Primer Start | 943996 | Primer End | 944228 |
| FORWARD_PRIMER_3 | |||||||||||||
| GTCGGTCTCACAGCTTCGTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTACCTCCGGAAATAAGGCA | |||||||||||||
| Tm_F_3 | 60.453 | Size_F_3 | 20 | Tm_R_3 | 59.542 | Size_R_3 | 20 | PRODUCT Size | 220 bp | Primer Start | 944009 | Primer End | 944228 |