Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(TTTC)3
Start:494247 End:494258
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTCCCTCACCTTCTCTCACG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GAAGAGCGAAGCAATGAACC | |||||||||||||
| Tm_F_1 | 59.978 | Size_F_1 | 20 | Tm_R_1 | 59.962 | Size_R_1 | 20 | PRODUCT Size | 236 bp | Primer Start | 494113 | Primer End | 494348 |
| FORWARD_PRIMER_2 | |||||||||||||
| CTCCCTCACCTTCTCTCACG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCGAATGAGTGAAGCAATGA | |||||||||||||
| Tm_F_2 | 59.978 | Size_F_2 | 20 | Tm_R_2 | 59.955 | Size_R_2 | 20 | PRODUCT Size | 201 bp | Primer Start | 494113 | Primer End | 494313 |
| FORWARD_PRIMER_3 | |||||||||||||
| CTCCCTCACCTTCTCTCACG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGACACACAACGAATGAGCG | |||||||||||||
| Tm_F_3 | 59.978 | Size_F_3 | 20 | Tm_R_3 | 59.905 | Size_R_3 | 20 | PRODUCT Size | 279 bp | Primer Start | 494113 | Primer End | 494391 |