Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(TTTC)3
Start:488580 End:488591
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTTGTTCCGGTTCTGGTTTC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGTAGGGCTAGGGCGATTGT | |||||||||||||
| Tm_F_1 | 59.948 | Size_F_1 | 20 | Tm_R_1 | 60.117 | Size_R_1 | 20 | PRODUCT Size | 186 bp | Primer Start | 488550 | Primer End | 488735 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCCAGTTTTTGTTCCGGTTC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AGTAGGGCTAGGGCGATTGT | |||||||||||||
| Tm_F_2 | 59.948 | Size_F_2 | 20 | Tm_R_2 | 60.117 | Size_R_2 | 20 | PRODUCT Size | 193 bp | Primer Start | 488543 | Primer End | 488735 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCACCTCCTCCTTTGTTTGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGTAGGGCTAGGGCGATTGT | |||||||||||||
| Tm_F_3 | 60.081 | Size_F_3 | 20 | Tm_R_3 | 60.117 | Size_R_3 | 20 | PRODUCT Size | 261 bp | Primer Start | 488475 | Primer End | 488735 |