Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(TTGT)3
Start:537228 End:537239
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCCTTTCTTTCAGTATGTATTGTG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCGGAACCCTCTTTTCTTTT | |||||||||||||
| Tm_F_1 | 57.011 | Size_F_1 | 24 | Tm_R_1 | 59.704 | Size_R_1 | 20 | PRODUCT Size | 273 bp | Primer Start | 537157 | Primer End | 537429 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCTTTTGAGTATATGGTTACCTTTTGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCGGAACCCTCTTTTCTTTT | |||||||||||||
| Tm_F_2 | 60.067 | Size_F_2 | 27 | Tm_R_2 | 59.704 | Size_R_2 | 20 | PRODUCT Size | 239 bp | Primer Start | 537191 | Primer End | 537429 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCTTTTGAGTATATGGTTACCTTTTGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TAGATAGCTAGCGCGGAACC | |||||||||||||
| Tm_F_3 | 60.067 | Size_F_3 | 27 | Tm_R_3 | 59.614 | Size_R_3 | 20 | PRODUCT Size | 251 bp | Primer Start | 537191 | Primer End | 537441 |