Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(TTGG)4
Start:6122 End:6137
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ATTCCCCTGGCTCGTAAACT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGTAGCTCCGACGACTGGAA | |||||||||||||
| Tm_F_1 | 59.962 | Size_F_1 | 20 | Tm_R_1 | 60.012 | Size_R_1 | 20 | PRODUCT Size | 225 bp | Primer Start | 6072 | Primer End | 6296 |
| FORWARD_PRIMER_2 | |||||||||||||
| ATTCCCCTGGCTCGTAAACT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CAATAGGCTCTCCAAGCACC | |||||||||||||
| Tm_F_2 | 59.962 | Size_F_2 | 20 | Tm_R_2 | 59.836 | Size_R_2 | 20 | PRODUCT Size | 262 bp | Primer Start | 6072 | Primer End | 6333 |
| FORWARD_PRIMER_3 | |||||||||||||
| TACCTCATTCATTCCCCTGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGTAGCTCCGACGACTGGAA | |||||||||||||
| Tm_F_3 | 59.744 | Size_F_3 | 20 | Tm_R_3 | 60.012 | Size_R_3 | 20 | PRODUCT Size | 235 bp | Primer Start | 6062 | Primer End | 6296 |