Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(TTGG)3
Start:489095 End:489106
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTCAATCGAGTCCCGTTTTC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ATGTCCCCCTCCACCTTTAC | |||||||||||||
| Tm_F_1 | 60.051 | Size_F_1 | 20 | Tm_R_1 | 60.052 | Size_R_1 | 20 | PRODUCT Size | 159 bp | Primer Start | 489061 | Primer End | 489219 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTCAATCGAGTCCCGTTTTC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GTATGTCCCCCTCCACCTTT | |||||||||||||
| Tm_F_2 | 60.051 | Size_F_2 | 20 | Tm_R_2 | 60.052 | Size_R_2 | 20 | PRODUCT Size | 161 bp | Primer Start | 489061 | Primer End | 489221 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCGAGATGAGTTCTTGCCAT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATGTCCCCCTCCACCTTTAC | |||||||||||||
| Tm_F_3 | 60.218 | Size_F_3 | 20 | Tm_R_3 | 60.052 | Size_R_3 | 20 | PRODUCT Size | 232 bp | Primer Start | 488988 | Primer End | 489219 |