Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(TTCT)3ttcccgaactgtaaacaagggaaataggctggattggatgagaagcgactgaaccgtcacctatttcttctttttcttctcttgatcctgaac(TCAA)3
Start:135166 End:135282
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GGTCAAGCTATTCCATTGCC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGTAAAGGACAGGCGAAGGC | |||||||||||||
| Tm_F_1 | 59.533 | Size_F_1 | 20 | Tm_R_1 | 60.763 | Size_R_1 | 20 | PRODUCT Size | 268 bp | Primer Start | 135089 | Primer End | 135356 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGTCAAGCTATTCCATTGCC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GTAAAGGACAGGCGAAGGC | |||||||||||||
| Tm_F_2 | 59.533 | Size_F_2 | 20 | Tm_R_2 | 59.828 | Size_R_2 | 19 | PRODUCT Size | 267 bp | Primer Start | 135089 | Primer End | 135355 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGAGGATCTGAAGGTCAAGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGTAAAGGACAGGCGAAGGC | |||||||||||||
| Tm_F_3 | 58.818 | Size_F_3 | 20 | Tm_R_3 | 60.763 | Size_R_3 | 20 | PRODUCT Size | 280 bp | Primer Start | 135077 | Primer End | 135356 |