Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(TTCT)3tcttttttagaaccaagtccttgaagccataataccaa(TTATG)3
Start:390934 End:390998
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACGTTCTTTGCTTACACCCG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GACTGTGGCTATACCGAGCC | |||||||||||||
| Tm_F_1 | 60.168 | Size_F_1 | 20 | Tm_R_1 | 59.723 | Size_R_1 | 20 | PRODUCT Size | 194 bp | Primer Start | 390889 | Primer End | 391082 |
| FORWARD_PRIMER_2 | |||||||||||||
| ACGTTCTTTGCTTACACCCG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ATACCGAGCCCCATCTTTTC | |||||||||||||
| Tm_F_2 | 60.168 | Size_F_2 | 20 | Tm_R_2 | 60.286 | Size_R_2 | 20 | PRODUCT Size | 184 bp | Primer Start | 390889 | Primer End | 391072 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTTTGAATTTCCCACGCTCT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GACTGTGGCTATACCGAGCC | |||||||||||||
| Tm_F_3 | 59.685 | Size_F_3 | 20 | Tm_R_3 | 59.723 | Size_R_3 | 20 | PRODUCT Size | 254 bp | Primer Start | 390829 | Primer End | 391082 |