Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(TTCT)3
Start:830724 End:830735
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCCTTTTACTCGGGTGGAAC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCGAAATCAGGGTCAGAAGT | |||||||||||||
| Tm_F_1 | 60.714 | Size_F_1 | 20 | Tm_R_1 | 59.288 | Size_R_1 | 20 | PRODUCT Size | 278 bp | Primer Start | 830676 | Primer End | 830953 |
| FORWARD_PRIMER_2 | |||||||||||||
| AACCTTTACTCCTGCACCGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCTTTGACAAGGGAGAGTCCA | |||||||||||||
| Tm_F_2 | 59.734 | Size_F_2 | 20 | Tm_R_2 | 59.829 | Size_R_2 | 21 | PRODUCT Size | 256 bp | Primer Start | 830547 | Primer End | 830802 |
| FORWARD_PRIMER_3 | |||||||||||||
| CGAACCTTTACTCCTGCACC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCTTTGACAAGGGAGAGTCCA | |||||||||||||
| Tm_F_3 | 59.734 | Size_F_3 | 20 | Tm_R_3 | 59.829 | Size_R_3 | 21 | PRODUCT Size | 258 bp | Primer Start | 830545 | Primer End | 830802 |