Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(TTCT)3
Start:78551 End:78562
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GCTATGTCTTCCTTCCGTGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CTCGCTCCAGAAATACGAGG | |||||||||||||
| Tm_F_1 | 59.694 | Size_F_1 | 20 | Tm_R_1 | 59.971 | Size_R_1 | 20 | PRODUCT Size | 270 bp | Primer Start | 78424 | Primer End | 78693 |
| FORWARD_PRIMER_2 | |||||||||||||
| GCTATGTCTTCCTTCCGTGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GATACCATTCTTCTGGGCGA | |||||||||||||
| Tm_F_2 | 59.694 | Size_F_2 | 20 | Tm_R_2 | 60.036 | Size_R_2 | 20 | PRODUCT Size | 235 bp | Primer Start | 78424 | Primer End | 78658 |
| FORWARD_PRIMER_3 | |||||||||||||
| ATGTCTTCCTTCCGTGGAGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CTCGCTCCAGAAATACGAGG | |||||||||||||
| Tm_F_3 | 59.655 | Size_F_3 | 20 | Tm_R_3 | 59.971 | Size_R_3 | 20 | PRODUCT Size | 267 bp | Primer Start | 78427 | Primer End | 78693 |