Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(TTCT)3
Start:632357 End:632368
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTGGAAACTCGGGATCGTAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ATTCTGGTCCTGGGAGAGGT | |||||||||||||
| Tm_F_1 | 60.066 | Size_F_1 | 20 | Tm_R_1 | 59.929 | Size_R_1 | 20 | PRODUCT Size | 265 bp | Primer Start | 632253 | Primer End | 632517 |
| FORWARD_PRIMER_2 | |||||||||||||
| CTGGAAACTCGGGATCGTAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AGGGGCTTTGCTCCTACAAT | |||||||||||||
| Tm_F_2 | 60.066 | Size_F_2 | 20 | Tm_R_2 | 60.096 | Size_R_2 | 20 | PRODUCT Size | 139 bp | Primer Start | 632253 | Primer End | 632391 |
| FORWARD_PRIMER_3 | |||||||||||||
| GAAGTGCGATGTGTCTGGAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATTCTGGTCCTGGGAGAGGT | |||||||||||||
| Tm_F_3 | 59.837 | Size_F_3 | 20 | Tm_R_3 | 59.929 | Size_R_3 | 20 | PRODUCT Size | 279 bp | Primer Start | 632239 | Primer End | 632517 |