Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(TTCT)3
Start:548560 End:548571
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GCAGGGTAGCTGACTTGCTC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CGTCCAATCCTCTACGCATT | |||||||||||||
| Tm_F_1 | 60.164 | Size_F_1 | 20 | Tm_R_1 | 60.096 | Size_R_1 | 20 | PRODUCT Size | 171 bp | Primer Start | 548520 | Primer End | 548690 |
| FORWARD_PRIMER_2 | |||||||||||||
| GCAGGGTAGCTGACTTGCTC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CGCCTTCGACTTCAGTAACC | |||||||||||||
| Tm_F_2 | 60.164 | Size_F_2 | 20 | Tm_R_2 | 59.875 | Size_R_2 | 20 | PRODUCT Size | 141 bp | Primer Start | 548520 | Primer End | 548660 |
| FORWARD_PRIMER_3 | |||||||||||||
| GCAGGGTAGCTGACTTGCTC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCGCCTTCGACTTCAGTAAC | |||||||||||||
| Tm_F_3 | 60.164 | Size_F_3 | 20 | Tm_R_3 | 59.875 | Size_R_3 | 20 | PRODUCT Size | 142 bp | Primer Start | 548520 | Primer End | 548661 |