Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(TTCT)3
Start:396142 End:396153
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTCGCGCTAAGTTACGATCC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AAGCTCCTTCTTCTTTCGCC | |||||||||||||
| Tm_F_1 | 60.003 | Size_F_1 | 20 | Tm_R_1 | 60.096 | Size_R_1 | 20 | PRODUCT Size | 269 bp | Primer Start | 395956 | Primer End | 396224 |
| FORWARD_PRIMER_2 | |||||||||||||
| GAAGGGGAAAAATAGTGGGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTCATTCTCGTTCGGCTCTT | |||||||||||||
| Tm_F_2 | 59.777 | Size_F_2 | 20 | Tm_R_2 | 59.955 | Size_R_2 | 20 | PRODUCT Size | 262 bp | Primer Start | 395991 | Primer End | 396252 |
| FORWARD_PRIMER_3 | |||||||||||||
| CTCGCGCTAAGTTACGATCC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGGTCCAAAAGCTCCTTCTT | |||||||||||||
| Tm_F_3 | 60.003 | Size_F_3 | 20 | Tm_R_3 | 59.691 | Size_R_3 | 20 | PRODUCT Size | 277 bp | Primer Start | 395956 | Primer End | 396232 |