Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(TTCT)3
Start:312197 End:312208
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCGAGCTCGCTTTAGCTTTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCCTTCGCTACTGAACGAAC | |||||||||||||
| Tm_F_1 | 61.109 | Size_F_1 | 20 | Tm_R_1 | 59.875 | Size_R_1 | 20 | PRODUCT Size | 274 bp | Primer Start | 312164 | Primer End | 312437 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCGAGCTCGCTTTAGCTTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCCTTCGCTACTGAACGAAC | |||||||||||||
| Tm_F_2 | 59.875 | Size_F_2 | 19 | Tm_R_2 | 59.875 | Size_R_2 | 20 | PRODUCT Size | 274 bp | Primer Start | 312164 | Primer End | 312437 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGAACCTTCGCCTTCACTCT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TAATCAATGGGCCTTACACG | |||||||||||||
| Tm_F_3 | 59.989 | Size_F_3 | 20 | Tm_R_3 | 58.506 | Size_R_3 | 20 | PRODUCT Size | 117 bp | Primer Start | 312115 | Primer End | 312231 |