Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(TTCCC)3ctgcccctgtattccctgattattcttgaactgtaaaagatcaatactctac(TCCT)3
Start:872817 End:872895
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGCTTTCACTGATCTTTTCGC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CTCGAAAGAAGAGGCAATCG | |||||||||||||
| Tm_F_1 | 60.517 | Size_F_1 | 21 | Tm_R_1 | 60.088 | Size_R_1 | 20 | PRODUCT Size | 260 bp | Primer Start | 872766 | Primer End | 873025 |
| FORWARD_PRIMER_2 | |||||||||||||
| GCTCTTTCCTTCACCTCTGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GAAGCTGTGAGACCGAGTGA | |||||||||||||
| Tm_F_2 | 59.166 | Size_F_2 | 20 | Tm_R_2 | 59.129 | Size_R_2 | 20 | PRODUCT Size | 275 bp | Primer Start | 872722 | Primer End | 872996 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCTTTTCGCCTCTCCCTATTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CTCGAAAGAAGAGGCAATCG | |||||||||||||
| Tm_F_3 | 59.342 | Size_F_3 | 21 | Tm_R_3 | 60.088 | Size_R_3 | 20 | PRODUCT Size | 248 bp | Primer Start | 872778 | Primer End | 873025 |