Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(TTCA)4ttctttctcct(TAAC)3tactttgct(ACTA)3
Start:385290 End:385349
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCTGCTTCACGCCTTACCTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ACCCTTTACCGACGAGGAGT | |||||||||||||
| Tm_F_1 | 60.015 | Size_F_1 | 20 | Tm_R_1 | 59.994 | Size_R_1 | 20 | PRODUCT Size | 197 bp | Primer Start | 385205 | Primer End | 385401 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCTGCTTCACGCCTTACCTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GAAGCCCCATATCCATCCTT | |||||||||||||
| Tm_F_2 | 60.015 | Size_F_2 | 20 | Tm_R_2 | 60.117 | Size_R_2 | 20 | PRODUCT Size | 223 bp | Primer Start | 385205 | Primer End | 385427 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCTGCTTCACGCCTTACCTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AAGAAGCCCCATATCCATCC | |||||||||||||
| Tm_F_3 | 60.015 | Size_F_3 | 20 | Tm_R_3 | 60.117 | Size_R_3 | 20 | PRODUCT Size | 225 bp | Primer Start | 385205 | Primer End | 385429 |