Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(TTCA)4
Start:852955 End:852970
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CGGAGCTAGCTAAGCCACTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CACCTTTTCGCTACTGAGCC | |||||||||||||
| Tm_F_1 | 58.906 | Size_F_1 | 20 | Tm_R_1 | 60.015 | Size_R_1 | 20 | PRODUCT Size | 235 bp | Primer Start | 852795 | Primer End | 853029 |
| FORWARD_PRIMER_2 | |||||||||||||
| CGGAGCTAGCTAAGCCACTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AAAGCGTCACCTTTTCGCTA | |||||||||||||
| Tm_F_2 | 58.906 | Size_F_2 | 20 | Tm_R_2 | 60.018 | Size_R_2 | 20 | PRODUCT Size | 242 bp | Primer Start | 852795 | Primer End | 853036 |
| FORWARD_PRIMER_3 | |||||||||||||
| CGGAGCTAGCTAAGCCACTTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CACCTTTTCGCTACTGAGCC | |||||||||||||
| Tm_F_3 | 60.176 | Size_F_3 | 21 | Tm_R_3 | 60.015 | Size_R_3 | 20 | PRODUCT Size | 235 bp | Primer Start | 852795 | Primer End | 853029 |