Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(TTC)5tttttcggaaggctag(ATATA)3
Start:645514 End:645559
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CGCTCCTCTTGTTACGTGGT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GAGTGTTTGAAGGCAGAGGC | |||||||||||||
| Tm_F_1 | 60.312 | Size_F_1 | 20 | Tm_R_1 | 59.997 | Size_R_1 | 20 | PRODUCT Size | 234 bp | Primer Start | 645484 | Primer End | 645717 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGGAACTCGGTAAAGCGTCT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AGCGAGACCGAGTGAAAGAG | |||||||||||||
| Tm_F_2 | 59.875 | Size_F_2 | 20 | Tm_R_2 | 59.745 | Size_R_2 | 20 | PRODUCT Size | 272 bp | Primer Start | 645463 | Primer End | 645734 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGGAACTCGGTAAAGCGTCT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCAATCTGGTTCCGGATCAT | |||||||||||||
| Tm_F_3 | 59.875 | Size_F_3 | 20 | Tm_R_3 | 60.281 | Size_R_3 | 20 | PRODUCT Size | 175 bp | Primer Start | 645463 | Primer End | 645637 |