Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(TTC)4cggttcaggcgcttctcttcctgttctttcattc(TCT)5
Start:778277 End:778337
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCGGTTCAGTCGAGAAAAGC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGATTCTTGAACTCAGGTGGG | |||||||||||||
| Tm_F_1 | 60.517 | Size_F_1 | 20 | Tm_R_1 | 60.096 | Size_R_1 | 21 | PRODUCT Size | 263 bp | Primer Start | 778209 | Primer End | 778471 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCGGTTCAGTCGAGAAAAGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGAACTCAATGAGGGAGAGGA | |||||||||||||
| Tm_F_2 | 60.517 | Size_F_2 | 20 | Tm_R_2 | 59.788 | Size_R_2 | 21 | PRODUCT Size | 170 bp | Primer Start | 778209 | Primer End | 778378 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCGGTTCAGTCGAGAAAAGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CGAACTGAACTCAATGAGGGA | |||||||||||||
| Tm_F_3 | 60.517 | Size_F_3 | 20 | Tm_R_3 | 60.239 | Size_R_3 | 21 | PRODUCT Size | 175 bp | Primer Start | 778209 | Primer End | 778383 |