Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(TTAC)4
Start:214442 End:214457
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCGGGATCATAAAGTCAGGC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCTCCGGAAACTGATTCAAG | |||||||||||||
| Tm_F_1 | 60.036 | Size_F_1 | 20 | Tm_R_1 | 59.813 | Size_R_1 | 20 | PRODUCT Size | 272 bp | Primer Start | 214255 | Primer End | 214526 |
| FORWARD_PRIMER_2 | |||||||||||||
| CAGGCTGACGAAGAGGAAAG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCTCCGGAAACTGATTCAAG | |||||||||||||
| Tm_F_2 | 60.126 | Size_F_2 | 20 | Tm_R_2 | 59.813 | Size_R_2 | 20 | PRODUCT Size | 257 bp | Primer Start | 214270 | Primer End | 214526 |
| FORWARD_PRIMER_3 | |||||||||||||
| CAGGCTGACGAAGAGGAAAG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACCTCAGTCTCTCAGCTCGG | |||||||||||||
| Tm_F_3 | 60.126 | Size_F_3 | 20 | Tm_R_3 | 59.732 | Size_R_3 | 20 | PRODUCT Size | 276 bp | Primer Start | 214270 | Primer End | 214545 |