Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(TGGT)3
Start:340728 End:340739
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCGTGGTCAAAATGTCAACTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCCAGCCTTTGTCCTAATCA | |||||||||||||
| Tm_F_1 | 59.883 | Size_F_1 | 21 | Tm_R_1 | 60.066 | Size_R_1 | 20 | PRODUCT Size | 270 bp | Primer Start | 340665 | Primer End | 340934 |
| FORWARD_PRIMER_2 | |||||||||||||
| ATCCCGTGGTCAAAATGTCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCCAGCCTTTGTCCTAATCA | |||||||||||||
| Tm_F_2 | 61.168 | Size_F_2 | 20 | Tm_R_2 | 60.066 | Size_R_2 | 20 | PRODUCT Size | 273 bp | Primer Start | 340662 | Primer End | 340934 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGATTATCCCGTGGTCAAAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCCAGCCTTTGTCCTAATCA | |||||||||||||
| Tm_F_3 | 58.818 | Size_F_3 | 20 | Tm_R_3 | 60.066 | Size_R_3 | 20 | PRODUCT Size | 278 bp | Primer Start | 340657 | Primer End | 340934 |