Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(TGAA)3
Start:221438 End:221449
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GGCCGGAGTTCAGTTCTACA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTTGCTCCTTCCCCTATTGA | |||||||||||||
| Tm_F_1 | 60.255 | Size_F_1 | 20 | Tm_R_1 | 59.64 | Size_R_1 | 20 | PRODUCT Size | 257 bp | Primer Start | 221311 | Primer End | 221567 |
| FORWARD_PRIMER_2 | |||||||||||||
| AAGAATCCATAGGTTCCGGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTTGCTCCTTCCCCTATTGA | |||||||||||||
| Tm_F_2 | 60.286 | Size_F_2 | 20 | Tm_R_2 | 59.64 | Size_R_2 | 20 | PRODUCT Size | 274 bp | Primer Start | 221294 | Primer End | 221567 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGCCGGAGTTCAGTTCTACA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CAAGATTTGCTCCTTCCCCT | |||||||||||||
| Tm_F_3 | 60.255 | Size_F_3 | 20 | Tm_R_3 | 60.569 | Size_R_3 | 20 | PRODUCT Size | 262 bp | Primer Start | 221311 | Primer End | 221572 |