Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(TCTTT)3
Start:164333 End:164347
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGAAGCTGAAGCTGTTAGGGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCTCTATGACCTGACCGGAA | |||||||||||||
| Tm_F_1 | 60.139 | Size_F_1 | 21 | Tm_R_1 | 60.065 | Size_R_1 | 20 | PRODUCT Size | 235 bp | Primer Start | 164215 | Primer End | 164449 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGAAGCTGAAGCTGTTAGGGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCTATGACCTGACCGGAACC | |||||||||||||
| Tm_F_2 | 60.139 | Size_F_2 | 21 | Tm_R_2 | 59.927 | Size_R_2 | 20 | PRODUCT Size | 233 bp | Primer Start | 164215 | Primer End | 164447 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGTCCGTCAGAGTTCAATCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCTCTATGACCTGACCGGAA | |||||||||||||
| Tm_F_3 | 58.643 | Size_F_3 | 20 | Tm_R_3 | 60.065 | Size_R_3 | 20 | PRODUCT Size | 161 bp | Primer Start | 164289 | Primer End | 164449 |