Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(TCTTG)3
Start:918547 End:918561
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CGGCGGTAAAGCATATGAAG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ACCGGAACCTATCCATCCTT | |||||||||||||
| Tm_F_1 | 60.599 | Size_F_1 | 20 | Tm_R_1 | 59.651 | Size_R_1 | 20 | PRODUCT Size | 167 bp | Primer Start | 918459 | Primer End | 918625 |
| FORWARD_PRIMER_2 | |||||||||||||
| CGGCGGTAAAGCATATGAAG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CTCACCGGAACCTATCCATC | |||||||||||||
| Tm_F_2 | 60.599 | Size_F_2 | 20 | Tm_R_2 | 59.361 | Size_R_2 | 20 | PRODUCT Size | 170 bp | Primer Start | 918459 | Primer End | 918628 |
| FORWARD_PRIMER_3 | |||||||||||||
| CGGCGGTAAAGCATATGAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCGGAACCTATCCATCCTTT | |||||||||||||
| Tm_F_3 | 59.671 | Size_F_3 | 19 | Tm_R_3 | 60.145 | Size_R_3 | 20 | PRODUCT Size | 166 bp | Primer Start | 918459 | Primer End | 918624 |