Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(TCTT)4
Start:295181 End:295196
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ATAGGGCGTCAACCCTTCTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AACGAGCACCGTGGAAATAC | |||||||||||||
| Tm_F_1 | 59.962 | Size_F_1 | 20 | Tm_R_1 | 60 | Size_R_1 | 20 | PRODUCT Size | 266 bp | Primer Start | 294969 | Primer End | 295234 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCCTTCATGCTGATGCTCAC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AACGAGCACCGTGGAAATAC | |||||||||||||
| Tm_F_2 | 59.95 | Size_F_2 | 20 | Tm_R_2 | 60 | Size_R_2 | 20 | PRODUCT Size | 157 bp | Primer Start | 295078 | Primer End | 295234 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTTGGAACCTTCCTTCATGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AACGAGCACCGTGGAAATAC | |||||||||||||
| Tm_F_3 | 60.051 | Size_F_3 | 20 | Tm_R_3 | 60 | Size_R_3 | 20 | PRODUCT Size | 167 bp | Primer Start | 295068 | Primer End | 295234 |