Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(TCTT)4
Start:280896 End:280911
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AATAGAGTGGACGGACGACG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTTATTGCGAGCCAAAAACC | |||||||||||||
| Tm_F_1 | 60.134 | Size_F_1 | 20 | Tm_R_1 | 60.075 | Size_R_1 | 20 | PRODUCT Size | 153 bp | Primer Start | 280795 | Primer End | 280947 |
| FORWARD_PRIMER_2 | |||||||||||||
| ATAGGGCGAGCAGCGTAATA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTTATTGCGAGCCAAAAACC | |||||||||||||
| Tm_F_2 | 59.859 | Size_F_2 | 20 | Tm_R_2 | 60.075 | Size_R_2 | 20 | PRODUCT Size | 169 bp | Primer Start | 280779 | Primer End | 280947 |
| FORWARD_PRIMER_3 | |||||||||||||
| GTGAGAGCCCAGACCATAGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCCATTCACAAGGACTCGAT | |||||||||||||
| Tm_F_3 | 59.834 | Size_F_3 | 20 | Tm_R_3 | 59.927 | Size_R_3 | 20 | PRODUCT Size | 264 bp | Primer Start | 280818 | Primer End | 281081 |