Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(TCTT)3
Start:571491 End:571502
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GCTTAAGCCAAGATGCGTTC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ACCAAAGCTAAGGTGCAAGC | |||||||||||||
| Tm_F_1 | 59.988 | Size_F_1 | 20 | Tm_R_1 | 59.522 | Size_R_1 | 20 | PRODUCT Size | 182 bp | Primer Start | 571468 | Primer End | 571649 |
| FORWARD_PRIMER_2 | |||||||||||||
| GCTTAAGCCAAGATGCGTTC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ACAAATACCAAGCAAGCCCA | |||||||||||||
| Tm_F_2 | 59.988 | Size_F_2 | 20 | Tm_R_2 | 60.502 | Size_R_2 | 20 | PRODUCT Size | 135 bp | Primer Start | 571468 | Primer End | 571602 |
| FORWARD_PRIMER_3 | |||||||||||||
| GCTTAAGCCAAGATGCGTTC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CAAATACCAAGCAAGCCCAG | |||||||||||||
| Tm_F_3 | 59.988 | Size_F_3 | 20 | Tm_R_3 | 60.628 | Size_R_3 | 20 | PRODUCT Size | 134 bp | Primer Start | 571468 | Primer End | 571601 |