Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(TCT)4
Start:59295 End:59306
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTCCTTTGTTGCTTTCCCAC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CTTCGCTACGGACTTTGAGG | |||||||||||||
| Tm_F_1 | 60.088 | Size_F_1 | 20 | Tm_R_1 | 60.008 | Size_R_1 | 20 | PRODUCT Size | 130 bp | Primer Start | 59211 | Primer End | 59340 |
| FORWARD_PRIMER_2 | |||||||||||||
| TATTCTGGCTTGATGGGTCC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CTTCGCTACGGACTTTGAGG | |||||||||||||
| Tm_F_2 | 59.894 | Size_F_2 | 20 | Tm_R_2 | 60.008 | Size_R_2 | 20 | PRODUCT Size | 217 bp | Primer Start | 59124 | Primer End | 59340 |
| FORWARD_PRIMER_3 | |||||||||||||
| ATACGTCAGAATGCCCCAAC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CTTCGCTACGGACTTTGAGG | |||||||||||||
| Tm_F_3 | 59.82 | Size_F_3 | 20 | Tm_R_3 | 60.008 | Size_R_3 | 20 | PRODUCT Size | 195 bp | Primer Start | 59146 | Primer End | 59340 |