Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(TCCTAT)3
Start:725637 End:725654
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GACAAAAGAAAGGTTCGGTCC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCTCTATCGTCCTGCTTCGG | |||||||||||||
| Tm_F_1 | 59.969 | Size_F_1 | 21 | Tm_R_1 | 60.495 | Size_R_1 | 20 | PRODUCT Size | 256 bp | Primer Start | 725479 | Primer End | 725734 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGAGTATCCGTACCTCCCAAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CTGACCTAGGGCTGCTTCTG | |||||||||||||
| Tm_F_2 | 59.438 | Size_F_2 | 21 | Tm_R_2 | 60.149 | Size_R_2 | 20 | PRODUCT Size | 193 bp | Primer Start | 725596 | Primer End | 725788 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGAGTATCCGTACCTCCCAAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGGCTAGGAGAGGAGCGAAG | |||||||||||||
| Tm_F_3 | 59.438 | Size_F_3 | 21 | Tm_R_3 | 60.247 | Size_R_3 | 20 | PRODUCT Size | 273 bp | Primer Start | 725596 | Primer End | 725868 |