Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(TCAT)3
Start:516979 End:516990
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AGGGGAACCCCTTTACCTCT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ATGGATCACGGAAGAAGACG | |||||||||||||
| Tm_F_1 | 60.178 | Size_F_1 | 20 | Tm_R_1 | 60.073 | Size_R_1 | 20 | PRODUCT Size | 250 bp | Primer Start | 516766 | Primer End | 517015 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCTCCCTATCGGTCTCATGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ATGGATCACGGAAGAAGACG | |||||||||||||
| Tm_F_2 | 60.181 | Size_F_2 | 20 | Tm_R_2 | 60.073 | Size_R_2 | 20 | PRODUCT Size | 139 bp | Primer Start | 516877 | Primer End | 517015 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACTCGGTTTCATGTCTTCGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATGGATCACGGAAGAAGACG | |||||||||||||
| Tm_F_3 | 60.263 | Size_F_3 | 20 | Tm_R_3 | 60.073 | Size_R_3 | 20 | PRODUCT Size | 227 bp | Primer Start | 516789 | Primer End | 517015 |