Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(TCAAG)3
Start:936680 End:936694
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCCTATCGTATCGGCTTTCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AAGGATCGTCCAGCAGAGAA | |||||||||||||
| Tm_F_1 | 60.053 | Size_F_1 | 20 | Tm_R_1 | 59.95 | Size_R_1 | 20 | PRODUCT Size | 142 bp | Primer Start | 936603 | Primer End | 936744 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGGGAACTCCTCTCGTTACC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AAGGATCGTCCAGCAGAGAA | |||||||||||||
| Tm_F_2 | 59.935 | Size_F_2 | 20 | Tm_R_2 | 59.95 | Size_R_2 | 20 | PRODUCT Size | 191 bp | Primer Start | 936554 | Primer End | 936744 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGGCTCCTCTCCCTATCGTA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AAGGATCGTCCAGCAGAGAA | |||||||||||||
| Tm_F_3 | 59.79 | Size_F_3 | 20 | Tm_R_3 | 59.95 | Size_R_3 | 20 | PRODUCT Size | 152 bp | Primer Start | 936593 | Primer End | 936744 |