Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(TATG)3
Start:678995 End:679006
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACTCTCCGGAGGGGTAGGTA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GTGAGGGAATGCGTTACGTT | |||||||||||||
| Tm_F_1 | 59.95 | Size_F_1 | 20 | Tm_R_1 | 60 | Size_R_1 | 20 | PRODUCT Size | 161 bp | Primer Start | 678956 | Primer End | 679116 |
| FORWARD_PRIMER_2 | |||||||||||||
| ACTCTCCGGAGGGGTAGGTA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TAAAGGATCCCATTGAAGCG | |||||||||||||
| Tm_F_2 | 59.95 | Size_F_2 | 20 | Tm_R_2 | 60.031 | Size_R_2 | 20 | PRODUCT Size | 113 bp | Primer Start | 678956 | Primer End | 679068 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGGGTAGGTAATAGCCGGAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GTGAGGGAATGCGTTACGTT | |||||||||||||
| Tm_F_3 | 60.164 | Size_F_3 | 20 | Tm_R_3 | 60 | Size_R_3 | 20 | PRODUCT Size | 151 bp | Primer Start | 678966 | Primer End | 679116 |