Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(TATAG)3
Start:50080 End:50094
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGATCTTCGTCCAACAACGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CACCTTCATTCAGCCATGTG | |||||||||||||
| Tm_F_1 | 60.24 | Size_F_1 | 20 | Tm_R_1 | 60.112 | Size_R_1 | 20 | PRODUCT Size | 243 bp | Primer Start | 49935 | Primer End | 50177 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGGGCTTATTGAAATCGGAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CTGGAGGACAACCACCAACT | |||||||||||||
| Tm_F_2 | 60.395 | Size_F_2 | 20 | Tm_R_2 | 60.002 | Size_R_2 | 20 | PRODUCT Size | 224 bp | Primer Start | 50007 | Primer End | 50230 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTGGGCTTATTGAAATCGGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CTGGAGGACAACCACCAACT | |||||||||||||
| Tm_F_3 | 60.395 | Size_F_3 | 20 | Tm_R_3 | 60.002 | Size_R_3 | 20 | PRODUCT Size | 225 bp | Primer Start | 50006 | Primer End | 50230 |