Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(TAAG)3
Start:710227 End:710238
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCGATTCCAACGAATAAGGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ATCGGTTTCATTGCTCTGCT | |||||||||||||
| Tm_F_1 | 59.894 | Size_F_1 | 20 | Tm_R_1 | 59.843 | Size_R_1 | 20 | PRODUCT Size | 231 bp | Primer Start | 710088 | Primer End | 710318 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTCAGGCAATGGAATTGTGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCTCTCCCTCCAACAAGTCA | |||||||||||||
| Tm_F_2 | 60.049 | Size_F_2 | 20 | Tm_R_2 | 60.229 | Size_R_2 | 20 | PRODUCT Size | 252 bp | Primer Start | 710142 | Primer End | 710393 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGAGACCGACACAGGTTCAG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATCGGTTTCATTGCTCTGCT | |||||||||||||
| Tm_F_3 | 59.864 | Size_F_3 | 20 | Tm_R_3 | 59.843 | Size_R_3 | 20 | PRODUCT Size | 160 bp | Primer Start | 710159 | Primer End | 710318 |