Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(GTTT)4
Start:762988 End:763003
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCAGTCTTTCCTTCCGGTTC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCTCATATGGTTCCTCGTTCA | |||||||||||||
| Tm_F_1 | 59.259 | Size_F_1 | 20 | Tm_R_1 | 59.94 | Size_R_1 | 21 | PRODUCT Size | 158 bp | Primer Start | 762965 | Primer End | 763122 |
| FORWARD_PRIMER_2 | |||||||||||||
| CATCAGTCTTTCCTTCCGGT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCTCATATGGTTCCTCGTTCA | |||||||||||||
| Tm_F_2 | 59.137 | Size_F_2 | 20 | Tm_R_2 | 59.94 | Size_R_2 | 21 | PRODUCT Size | 160 bp | Primer Start | 762963 | Primer End | 763122 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCAGTCTTTCCTTCCGGTTC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACTCCACGTATTGCTTGACG | |||||||||||||
| Tm_F_3 | 59.259 | Size_F_3 | 20 | Tm_R_3 | 58.802 | Size_R_3 | 20 | PRODUCT Size | 257 bp | Primer Start | 762965 | Primer End | 763221 |