Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(GTTT)3
Start:615298 End:615309
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AAATGATTGAACAACCGGGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGGGTTTTCCATGAGATGGG | |||||||||||||
| Tm_F_1 | 60.17 | Size_F_1 | 20 | Tm_R_1 | 61.076 | Size_R_1 | 20 | PRODUCT Size | 166 bp | Primer Start | 615169 | Primer End | 615334 |
| FORWARD_PRIMER_2 | |||||||||||||
| GACGGACATTCCTTGCTCAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGCTAGGCTTGTTATCCAGG | |||||||||||||
| Tm_F_2 | 60.081 | Size_F_2 | 20 | Tm_R_2 | 58.812 | Size_R_2 | 20 | PRODUCT Size | 258 bp | Primer Start | 615234 | Primer End | 615491 |
| FORWARD_PRIMER_3 | |||||||||||||
| AATGATTGAACAACCGGGAG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGGGTTTTCCATGAGATGGG | |||||||||||||
| Tm_F_3 | 59.79 | Size_F_3 | 20 | Tm_R_3 | 61.076 | Size_R_3 | 20 | PRODUCT Size | 165 bp | Primer Start | 615170 | Primer End | 615334 |