Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(GTTC)3
Start:180355 End:180366
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ATCCGCACTACAACCAATCC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ACGGCTGTCAAAAGACGAGT | |||||||||||||
| Tm_F_1 | 59.82 | Size_F_1 | 20 | Tm_R_1 | 59.914 | Size_R_1 | 20 | PRODUCT Size | 226 bp | Primer Start | 180209 | Primer End | 180434 |
| FORWARD_PRIMER_2 | |||||||||||||
| ATCCGCACTACAACCAATCC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AGGATCACGGCTGTCAAAAG | |||||||||||||
| Tm_F_2 | 59.82 | Size_F_2 | 20 | Tm_R_2 | 60.255 | Size_R_2 | 20 | PRODUCT Size | 232 bp | Primer Start | 180209 | Primer End | 180440 |
| FORWARD_PRIMER_3 | |||||||||||||
| ATCCGCACTACAACCAATCC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CGGCATACGGAAAATCAATC | |||||||||||||
| Tm_F_3 | 59.82 | Size_F_3 | 20 | Tm_R_3 | 60.289 | Size_R_3 | 20 | PRODUCT Size | 253 bp | Primer Start | 180209 | Primer End | 180461 |