Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(GT)6
Start:306031 End:306042
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGACGTTCGAACCTACCTCC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGAAGATCATCTCAGCCATTTC | |||||||||||||
| Tm_F_1 | 60.111 | Size_F_1 | 20 | Tm_R_1 | 60.046 | Size_R_1 | 22 | PRODUCT Size | 198 bp | Primer Start | 305988 | Primer End | 306185 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGACGTTCGAACCTACCTCC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GAGTGCACAATAAATGCGAGA | |||||||||||||
| Tm_F_2 | 60.111 | Size_F_2 | 20 | Tm_R_2 | 58.934 | Size_R_2 | 21 | PRODUCT Size | 257 bp | Primer Start | 305988 | Primer End | 306244 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGACGTTCGAACCTACCTCC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGAGTGCACAATAAATGCGAGA | |||||||||||||
| Tm_F_3 | 60.111 | Size_F_3 | 20 | Tm_R_3 | 59.911 | Size_R_3 | 22 | PRODUCT Size | 258 bp | Primer Start | 305988 | Primer End | 306245 |