Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(GGTT)3
Start:576081 End:576092
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGTTGAGGTCTGTTTGCTCG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGGGGAAAGAAGGATCAGGA | |||||||||||||
| Tm_F_1 | 60.025 | Size_F_1 | 20 | Tm_R_1 | 60.008 | Size_R_1 | 20 | PRODUCT Size | 236 bp | Primer Start | 576022 | Primer End | 576257 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTCACGAACTGTCAACTCGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AGGGGAAAGAAGGATCAGGA | |||||||||||||
| Tm_F_2 | 59.873 | Size_F_2 | 20 | Tm_R_2 | 60.008 | Size_R_2 | 20 | PRODUCT Size | 208 bp | Primer Start | 576050 | Primer End | 576257 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTCACGAACTGTCAACTCGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CAATCCGGTGAAAAGGAGAA | |||||||||||||
| Tm_F_3 | 59.873 | Size_F_3 | 20 | Tm_R_3 | 60.044 | Size_R_3 | 20 | PRODUCT Size | 263 bp | Primer Start | 576050 | Primer End | 576312 |