Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(GGAA)3
Start:294232 End:294243
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTGTTGGTTGAGCCGTATGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ACGCCTTGTGATCTTCAACC | |||||||||||||
| Tm_F_1 | 60.111 | Size_F_1 | 20 | Tm_R_1 | 60.119 | Size_R_1 | 20 | PRODUCT Size | 232 bp | Primer Start | 294182 | Primer End | 294413 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCCATATGGTTTGGACAGCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCTGCAATTTCCGAATCTCC | |||||||||||||
| Tm_F_2 | 59.924 | Size_F_2 | 20 | Tm_R_2 | 60.155 | Size_R_2 | 20 | PRODUCT Size | 162 bp | Primer Start | 294148 | Primer End | 294309 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTGTTGGTTGAGCCGTATGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCTGCAATTTCCGAATCTCC | |||||||||||||
| Tm_F_3 | 60.111 | Size_F_3 | 20 | Tm_R_3 | 60.155 | Size_R_3 | 20 | PRODUCT Size | 128 bp | Primer Start | 294182 | Primer End | 294309 |