Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(GCTT)3
Start:647788 End:647799
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTCCGGAACAGGAAGTCAAG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AAAGCCAGCAAGCAAGAAAG | |||||||||||||
| Tm_F_1 | 59.837 | Size_F_1 | 20 | Tm_R_1 | 59.769 | Size_R_1 | 20 | PRODUCT Size | 191 bp | Primer Start | 647633 | Primer End | 647823 |
| FORWARD_PRIMER_2 | |||||||||||||
| CTCCGGAACAGGAAGTCAAG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AAAGCAAGCAAGAAAGCCAG | |||||||||||||
| Tm_F_2 | 59.837 | Size_F_2 | 20 | Tm_R_2 | 59.769 | Size_R_2 | 20 | PRODUCT Size | 203 bp | Primer Start | 647633 | Primer End | 647835 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACTCGGTTTCCTTTCCCAAT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AAAGCCAGCAAGCAAGAAAG | |||||||||||||
| Tm_F_3 | 59.805 | Size_F_3 | 20 | Tm_R_3 | 59.769 | Size_R_3 | 20 | PRODUCT Size | 278 bp | Primer Start | 647546 | Primer End | 647823 |