Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(GCAA)3
Start:778663 End:778674
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ATCGTATTAGGGGGACGGAC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GAGAGTTTACGGGACAGGCA | |||||||||||||
| Tm_F_1 | 60.039 | Size_F_1 | 20 | Tm_R_1 | 60.255 | Size_R_1 | 20 | PRODUCT Size | 244 bp | Primer Start | 778474 | Primer End | 778717 |
| FORWARD_PRIMER_2 | |||||||||||||
| CTCTCTGTCCTTCCAACCCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGTGGCAGGAAGACTCACTC | |||||||||||||
| Tm_F_2 | 60.229 | Size_F_2 | 20 | Tm_R_2 | 59.841 | Size_R_2 | 20 | PRODUCT Size | 188 bp | Primer Start | 778603 | Primer End | 778790 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCTCTGTCCTTCCAACCCAG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGTGGCAGGAAGACTCACTC | |||||||||||||
| Tm_F_3 | 60.229 | Size_F_3 | 20 | Tm_R_3 | 59.841 | Size_R_3 | 20 | PRODUCT Size | 187 bp | Primer Start | 778604 | Primer End | 778790 |