Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(GCAA)3
Start:40215 End:40226
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCCACCAATCGATCTTCTGT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CAACTTGAACGAGCCTTTCC | |||||||||||||
| Tm_F_1 | 59.927 | Size_F_1 | 20 | Tm_R_1 | 59.853 | Size_R_1 | 20 | PRODUCT Size | 276 bp | Primer Start | 40114 | Primer End | 40389 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCCACCAATCGATCTTCTGT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGGTCAAGTGCTTCCTTTCC | |||||||||||||
| Tm_F_2 | 59.927 | Size_F_2 | 20 | Tm_R_2 | 60.232 | Size_R_2 | 20 | PRODUCT Size | 244 bp | Primer Start | 40114 | Primer End | 40357 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTCCCACCAATCGATCTTCT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CAACTTGAACGAGCCTTTCC | |||||||||||||
| Tm_F_3 | 59.483 | Size_F_3 | 20 | Tm_R_3 | 59.853 | Size_R_3 | 20 | PRODUCT Size | 278 bp | Primer Start | 40112 | Primer End | 40389 |