Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(GAT)4
Start:781068 End:781079
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GCTTTCCGAGTGCTTGAATC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGGGAGACTCCAGCAAGACA | |||||||||||||
| Tm_F_1 | 59.962 | Size_F_1 | 20 | Tm_R_1 | 59.986 | Size_R_1 | 20 | PRODUCT Size | 189 bp | Primer Start | 780935 | Primer End | 781123 |
| FORWARD_PRIMER_2 | |||||||||||||
| GCTTTCCGAGTGCTTGAATC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CTCAACCGACACCCTTTGAT | |||||||||||||
| Tm_F_2 | 59.962 | Size_F_2 | 20 | Tm_R_2 | 59.966 | Size_R_2 | 20 | PRODUCT Size | 262 bp | Primer Start | 780935 | Primer End | 781196 |
| FORWARD_PRIMER_3 | |||||||||||||
| GCTTTCCGAGTGCTTGAATC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCCTTTGATGGAAGAGGTCA | |||||||||||||
| Tm_F_3 | 59.962 | Size_F_3 | 20 | Tm_R_3 | 60.042 | Size_R_3 | 20 | PRODUCT Size | 251 bp | Primer Start | 780935 | Primer End | 781185 |