Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(GAGT)3
Start:456406 End:456417
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCCCGTTTTGGTTTTATTGC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ACGTAAACGGTTGGTTGAGC | |||||||||||||
| Tm_F_1 | 59.81 | Size_F_1 | 20 | Tm_R_1 | 60.037 | Size_R_1 | 20 | PRODUCT Size | 137 bp | Primer Start | 456332 | Primer End | 456468 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCCCGTTTTGGTTTTATTGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AACGGTTGGTTGAGCTTGAG | |||||||||||||
| Tm_F_2 | 59.81 | Size_F_2 | 20 | Tm_R_2 | 60.291 | Size_R_2 | 20 | PRODUCT Size | 132 bp | Primer Start | 456332 | Primer End | 456463 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGCTTTATCCCGTTTTGGTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACGTAAACGGTTGGTTGAGC | |||||||||||||
| Tm_F_3 | 59.451 | Size_F_3 | 20 | Tm_R_3 | 60.037 | Size_R_3 | 20 | PRODUCT Size | 144 bp | Primer Start | 456325 | Primer End | 456468 |